E2F1 Antibody
Rabbit Polyclonal
Catalog number: 12171-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HeLa cells, A431 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP(E2F1 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human breast cancer using 12171-1-AP(E2F1 Antibody) at Dilution 1:50 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | E2F1 fusion protein ag2813 | Full name | E2F transcription factor 1 |
|
Calculated molecular weight |
437aa,47kd |
Observed molecular
weight |
55kd |
|
GenBank accession number |
BC050369 | Gene ID (NCBI) | 1869 |
| Gene symbol | E2F1 | Synonyms | E2F-1; RBAP1; RBBP3; RBP3 |
Background
DNA tumor viruses appear to have transforming activity due to their binding and inactivating to the RB1 gene products, and transcription facotr E2F, which is first identified as a DNA-binding protein essential for E1A-dependent activation of the advenovirus E2 promoter. The complex DRTF1/E2F involves in the control of cell-cycle progression from G1 to S phase. E2F1, one of E2F preferentially binds to RB1 protein in a cell-cycle dependent manner. It mediates both cell proliferation and p53-dependent apoptosis. |
