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Tested Applications
| Positive WB detected in | HepG2 cells, mouse skeletal muscle tissue, U-251 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
11241-1-AP targets EDEM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EDEM2 fusion protein Ag1755 Predict reactive species |
| Full Name | ER degradation enhancer, mannosidase alpha-like 2 |
| Calculated Molecular Weight | 64.8 kDa |
| Observed Molecular Weight | 60-65 kDa |
| GenBank Accession Number | BC016184 |
| Gene Symbol | EDEM2 |
| Gene ID (NCBI) | 55741 |
| RRID | AB_2877752 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BV94 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EDEM2 antibody 11241-1-AP | Download protocol |
| IHC protocol for EDEM2 antibody 11241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
