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EDEM2 Polyclonal antibody

EDEM2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11241-1-AP

Synonyms

C20orf31, C20orf49, EDEM2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EDEM2 Polyclonal antibody (11241-1-AP)

    WB analysis using 11241-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11241-1-AP (EDEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using EDEM2 Polyclonal antibody (11241-1-AP)

    WB analysis of mouse skeletal muscle using 11241-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11241-1-AP (EDEM2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human heart tissue using EDEM2 Polyclonal antibody (11241-1-AP)

    IHC staining of human heart using 11241-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11241-1-AP (EDEM2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using EDEM2 Polyclonal antibody (11241-1-AP)

    IHC staining of human heart using 11241-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11241-1-AP (EDEM2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue, U-251 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11241-1-AP targets EDEM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EDEM2 fusion protein Ag1755
Full Name ER degradation enhancer, mannosidase alpha-like 2
Calculated Molecular Weight64.8 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC016184
Gene Symbol EDEM2
Gene ID (NCBI) 55741
RRIDAB_2877752
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EDEM2 antibody 11241-1-APDownload protocol
IHC protocol for EDEM2 antibody 11241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols