EEF1B2 Antibody
Rabbit Polyclonal
Catalog number: 10095-2-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IF, IP, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | SKOV-3 cells, HEK-293 cells, HeLa cells, PC-3 cells, RAW264.7 cells |
| Positive IHC detected in | human pancreas cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP(EEF1B2 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human pancreas cancer using 10095-2-AP(EEF1B2 Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using 10095-2-AP and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | EEF1B2 fusion protein ag0135 | Full name | eukaryotic translation elongation factor 1 beta 2 |
|
Calculated molecular weight |
25kd |
Observed molecular
weight |
24-30 kDa, 34kDa |
|
GenBank accession number |
BC000211 | Gene ID (NCBI) | 1933 |
| Gene symbol | EEF1B2 | Synonyms | EEF1B; EEF1B1; EF1B |
Background
In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2. |
