EEF1E1 Antibody
Rabbit Polyclonal
Catalog number: 10805-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | human testis tissue, HEK-293 cells, HepG2 cells, Jurkat cells, SKOV-3 cells |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
human testis tissue were subjected to SDS PAGE followed by western blot with 10805-1-AP(EEF1E1 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human breast cancer using 10805-1-AP(EEF1E1 Antibody) at Dilution 1:10 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | EEF1E1 fusion protein ag1092 | Full name | eukaryotic translation elongation factor 1 epsilon 1 |
|
Calculated molecular weight |
20kd |
Observed molecular
weight |
20kd |
|
GenBank accession number |
BC005291 | Gene ID (NCBI) | 9521 |
| Gene symbol | EEF1E1 | Synonyms | AIMP3; P18 |
Background
During the translation processes, aminoacyl-tRNA synthetases (ARSs) ligate specific amino acids to tRNAs. Together with non-enzymatic proteins called ARS-interacting multifunctional proteins (AIMPs), the ARSs form a macromolecular complex [PMID:15286174]. This complex has an essential role in protein synthesis, but also in other biological processes including angiogenesis, apoptosis and inflammation. [PMID:21789020] AIMP3 is not only associated with the multi-tRNA synthetase complex via its specific interaction with methionyl-tRNA synthetase, but also works as a tumor suppressor by interacting with ATM, the upstream kinase of p53 [PMID:18343821]. |
