Tested Applications
| Positive WB detected in | HEK-293 cells, rat kidney tissue |
| Positive IHC detected in | human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
12999-1-AP targets Ephrin B1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3669 Product name: Recombinant human EFNB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-239 aa of BC016649 Sequence: LEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNRPEQEIRFTIKFQEFSPNYMGLEFKKHHDYYITSTSNGSLEGLENREGGVCRTRTMKIIMKVGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHETVNQEEKSGPGASGGSSGDPDGFFNSKVA Predict reactive species |
| Full Name | ephrin-B1 |
| Calculated Molecular Weight | 346 aa, 38 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC016649 |
| Gene Symbol | Ephrin B1 |
| Gene ID (NCBI) | 1947 |
| RRID | AB_10644156 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98172 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EFNB1 (Ephrin B1) is a member of the ephrin family, which are membrane-bound guidance cues that interact with Eph receptors, a family of receptor tyrosine kinases. EFNB1 is X-inactivated, and it has been proposed that in heterozygous females, patchy loss of ephrin B1 disturbs tissue boundary formation at the developing coronal suture (PMID: 34824583, 18627045). EFNB1 mainly plays a central part in cell adhesion, angiogenesis, and development of the nervous system (PMID: 30326247).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Ephrin B1 antibody 12999-1-AP | Download protocol |
| WB protocol for Ephrin B1 antibody 12999-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function. | ||
Signal Transduct Target Ther Aberrant epithelial cell interaction promotes esophageal squamous-cell carcinoma development and progression
| ||
