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  • Western Blot (WB) analysis of mouse brain tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis of mouse brain using 24729-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis using 24729-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    WB analysis of fetal human brain using 24729-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    IHC staining of human kidney using 24729-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EFTUD1 Polyclonal antibody (24729-1-AP)

    IHC staining of human kidney using 24729-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 40x lens).

EFTUD1 Polyclonal antibody

EFTUD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24729-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EFL1, Elongation factor Tu GTP-binding domain-containing protein 1, Elongation factor-like 1, Elongation factor-like GTPase 1, FAM42A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHeLa cells, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24729-1-AP targets EFTUD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20371

Product name: Recombinant human EFTUD1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 772-1120 aa of BC156743

Sequence: LIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Predict reactive species
Full Name elongation factor Tu GTP binding domain containing 1
Calculated Molecular Weight 1120 aa, 125 kDa
Observed Molecular Weight 125-135 kDa
GenBank Accession NumberBC156743
Gene Symbol EFTUD1
Gene ID (NCBI) 79631
RRIDAB_2879693
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z2Z2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EFTUD1 (Elongation Factor Tu GTP Binding Domain Containing Protein 1) is a protein involved in ribosome biogenesis and translation activation. It is a member of the GTP-binding elongation factor family. EFTUD1 plays a crucial role in the release of eukaryotic translation initiation factor 6 (eIF6) from 60S ribosome subunits, allowing these subunits to join with 40S subunits to form actively translating 80S complexes. (PMID: 25015090)

Protocols

Product Specific Protocols
IHC protocol for EFTUD1 antibody 24729-1-APDownload protocol
WB protocol for EFTUD1 antibody 24729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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