Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
| RIP | See 1 publications below |
Product Information
10208-1-AP targets EFTUD2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA, PLA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0279 Product name: Recombinant human EFTUD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 13-217 aa of BC002360 Sequence: IGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTA Predict reactive species |
| Full Name | elongation factor Tu GTP binding domain containing 2 |
| Calculated Molecular Weight | 116 kDa |
| Observed Molecular Weight | 116 kDa |
| GenBank Accession Number | BC002360 |
| Gene Symbol | EFTUD2 |
| Gene ID (NCBI) | 9343 |
| RRID | AB_2095834 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15029 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EFTUD2 encodes the 116 kDa U5 small nuclear ribonecleoprotein (snRNP) component, also named SNU114, it has GTP binding activity and is required for pre-mRNA splicing. Evolutionarily conserved human snRNP protein (U5-116kDa) is homologous to the ribosomal elongation factor EF-2 (ribosomal translocase). Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM). So far, acetylation and phosphorylation sites of EFTUD2 have been identified. Catolog#10208-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human EFTUD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EFTUD2 antibody 10208-1-AP | Download protocol |
| IP protocol for EFTUD2 antibody 10208-1-AP | Download protocol |
| WB protocol for EFTUD2 antibody 10208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol Syndromic immune disorder caused by a viable hypomorphic allele of spliceosome component Snrnp40. | ||
Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence. | ||
FASEB J Reduced prostaglandin I2 signaling in Arid5b-/- primary skeletal muscle cells attenuates myogenesis. | ||
Mol Cell Biol A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis. | ||
iScience Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells |
