EIF3D Antibody
Mouse Monoclonal
Catalog number: 66024-1-Ig
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human brain |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HepG2 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig(EIF3D Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human brain using 66024-1-Ig(EIF3D Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain using 66024-1-Ig(EIF3D Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Mouse | Purification method | Protein A purification |
| Isotype | IgG2b | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | EIF3D fusion protein ag18092 | Full name | eukaryotic translation initiation factor 3, subunit D |
|
Calculated molecular weight |
66kd |
Observed molecular
weight |
66kD |
|
GenBank accession number |
BC000328 | Gene ID (NCBI) | 8664 |
| Gene symbol | EIF3D | Synonyms | EIF3S7; MGC126526; MGC17258; eIF3-p66; eIF3-zeta |
Background
The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine. |
