EIF4A3 Antibody
Rabbit Polyclonal
Catalog number: 10463-1-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | IP, WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | human |
| Positive WB detected in | HEK-293 cells, HeLa cells, human liver tissue, human lung tissue, mouse liver tissue, Raji cells |
| Recommended dilution | WB: 1:200-1:1000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP(EIF4A3 Antibody) at dilution of 1:300
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | EIF4A3 fusion protein ag0733 | Full name | eukaryotic translation initiation factor 4A, isoform 3 |
|
Calculated molecular weight |
47kd |
Observed molecular
weight |
47kd |
|
GenBank accession number |
BC004386 | Gene ID (NCBI) | 9775 |
| Gene symbol | EIF4A3 | Synonyms | DDX48; KIAA0111; MGC10862; NMP265; NUK-34; eIF4AIII; hNMP265 |
Background
EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions |
