EIF4A3 Antibody
Rabbit Polyclonal
Catalog number: 17504-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, human placenta tissue, MCF7 cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse thymus tissue |
| Positive IHC detected in | human kidney |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP(EIF4A3 Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human kidney using 17504-1-AP(EIF4A3 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney using 17504-1-AP(EIF4A3 Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody 17504-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | EIF4A3 fusion protein ag11701 | Full name | eukaryotic translation initiation factor 4A, isoform 3 |
|
Calculated molecular weight |
47kd |
Observed molecular
weight |
47kd |
|
GenBank accession number |
BC003662 | Gene ID (NCBI) | 9775 |
| Gene symbol | EIF4A3 | Synonyms | DDX48; KIAA0111; MGC10862; NMP265; NUK-34; eIF4AIII; hNMP265 |
Background
EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions |
