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  • KD/KO Validated

EIF4G1 Polyclonal antibody

EIF4G1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15704-1-AP

Synonyms

eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220



Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells, Ethacrynic acid treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15704-1-AP targets EIF4G1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4G1 fusion protein Ag8342
Full Name eukaryotic translation initiation factor 4 gamma, 1
Calculated Molecular Weight 1600 aa, 176 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC007788
Gene Symbol EIF4G1
Gene ID (NCBI) 1981
RRIDAB_2261979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

Protocols

Product Specific Protocols
WB protocol for EIF4G1 antibody 15704-1-APDownload protocol
IHC protocol for EIF4G1 antibody 15704-1-APDownload protocol
IF protocol for EIF4G1 antibody 15704-1-APDownload protocol
IP protocol for EIF4G1 antibody 15704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
CoIP

Mol Cell

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Authors - Linyu Sun
humanIF

J Extracell Vesicles

Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation.

Authors - Bo You
humanWB

Proc Natl Acad Sci U S A

Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation.

Authors - Brian C Avanzino
humanWB

PLoS Genet

SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation.

Authors - Siqi Hu
mouseWB,IF

Diabetes

Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Authors - Seokwon Jo
  • KO Validated
humanIF

J Cell Sci

ADAR1 limits stress granule formation through both translation-dependent and translation-independent mechanisms.

Authors - Giulia A Corbet