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eIF4G2/DAP5 Polyclonal antibody

eIF4G2/DAP5 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17728-1-AP

Synonyms

AAG1, DAP 5, DAP5, Death associated protein 5, eIF 4 gamma 2, eIF 4G 2, eIF4G 2, eIF4G2, NAT1, p97

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    WB analysis using 17728-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    WB analysis of HeLa using 17728-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    WB analysis of MCF-7 using 17728-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using eIF4G2/DAP5 Polyclonal antibody (17728-1-AP)

    IP experiment of MCF-7 using 17728-1-AP

    IP result of anti-eIF4G2/DAP5 (IP:17728-1-AP, 4ug; Detection:17728-1-AP 1:400) with MCF-7 cells lysate 3200ug.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, Neuro-2a cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17728-1-AP targets eIF4G2/DAP5 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen eIF4G2/DAP5 fusion protein Ag12125
Full Name eukaryotic translation initiation factor 4 gamma, 2
Calculated Molecular Weight 907 aa, 102 kDa
Observed Molecular Weight 98-102 kDa
GenBank Accession NumberBC043149
Gene Symbol EIF4G2
Gene ID (NCBI) 1982
RRIDAB_2095895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for eIF4G2/DAP5 antibody 17728-1-APDownload protocol
IP protocol for eIF4G2/DAP5 antibody 17728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

PMID: 31792210

Authors - Sunwang Xu
View Article
humanWB

Shock

miR-379-5P INHIBITION ENHANCES INTESTINAL EPITHELIAL PROLIFERATION AND BARRIER FUNCTION RECOVERY AFTER ISCHEMIA/REPERFUSION BY TARGETING EIF4G2

Authors - Zirui Jia
View Article