Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:10-1:100 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
11281-1-AP targets EIF4G3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1811 Product name: Recombinant human EIF4G3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 197-515 aa of BC030578 Sequence: SQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC Predict reactive species |
| Full Name | eukaryotic translation initiation factor 4 gamma, 3 |
| Calculated Molecular Weight | 177 kDa |
| Observed Molecular Weight | 70 kDa, 250 kDa |
| GenBank Accession Number | BC030578 |
| Gene Symbol | EIF4G3 |
| Gene ID (NCBI) | 8672 |
| RRID | AB_2877754 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43432 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF4G3 antibody 11281-1-AP | Download protocol |
| IHC protocol for EIF4G3 antibody 11281-1-AP | Download protocol |
| IP protocol for EIF4G3 antibody 11281-1-AP | Download protocol |
| WB protocol for EIF4G3 antibody 11281-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia | ||
Jpn J Clin Oncol Identification of distinct genomic features reveals frequent somatic AHNAK and PTEN mutations predominantly in primary malignant melanoma presenting in the ureter. | ||
Cancer Res Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression | ||
Sci Adv High-throughput tagging of endogenous loci for rapid characterization of protein function | ||
Mol Biotechnol Expression Profile and Prognostic Significance of Pivotal Regulators for N7-Methylguanosine Methylation in Diffuse Large B-Cell Lymphoma | ||
Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control |
