EIF6 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | COLO 320 cells, HEK-293 cells, A375 cells, HeLa cells, HepG2 cells, mouse liver tissue |
| Positive IHC detected in | human prostate cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
IP = Immunoprecipitation
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10291-1-AP(EIF6 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate cancer using 10291-1-AP(EIF6 Antibody) at Dilution 1:50 (under 10x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | EIF6 fusion protein ag0324 | Full name | eukaryotic translation initiation factor 6 |
|
Calculated molecular weight |
27kd |
Observed molecular
weight |
27-30kd |
|
GenBank accession number |
BC001119 | Gene ID (NCBI) | 3692 |
| Gene symbol | EIF6 | Synonyms | 2; CAB; EIF3A; ITGB4BP; b; b(2)gcn; gcn; p27BBP |
p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), It functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage. |
