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HuC Polyclonal antibody
HuC Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, RIP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 55047-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, Neuro-2a cells |
| Positive IHC detected in | human lung cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 3 publications below |
| RIP | See 1 publications below |
Product Information
55047-1-AP targets HuC in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 36-38 kDa |
| GenBank Accession Number | NM_001420 |
| Gene Symbol | ELAVL3 |
| Gene ID (NCBI) | 1995 |
| RRID | AB_10859256 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HUC , belongs to the RRM elav family. It binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. ELAVL3 may also bind poly-A tracts via RRM 3. It is involved in neuronal differentiation and maintenance. The antibody has no cross reaction to other ELAVL members.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HuC antibody 55047-1-AP | Download protocol |
| IHC protocol for HuC antibody 55047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis. | ||
Am J Pathol Anaplastic lymphoma kinase (ALK) overexpression is associated with aggressive phenotypic characteristics of ovarian high-grade serous carcinoma. | ||
Cell Stem Cell KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury | ||
Front Cell Neurosci Choice of Alternative Polyadenylation Sites, Mediated by the RNA-Binding Protein Elavl3, Plays a Role in Differentiation of Inhibitory Neuronal Progenitors.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Etienne (Verified Customer) (12-19-2022) | Did not work. Virtually only aspecific binding in human brain organoids
 |
FH Alessandro (Verified Customer) (11-07-2022) | no unspecific staining, great outcome
|
FH David (Verified Customer) (09-01-2022) | Works good!
 |
