Tested Applications
| Positive IP detected in | K-562 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
16020-1-AP targets ELF4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8836 Product name: Recombinant human ELF4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-301 aa of BC017194 Sequence: MAITLQPSDLIFEFASNGMDDDIHQLEDPSVFPAVIVEQVPYPDLLHLYSGLELDDVHNGIITDGTLCMTQDQILEGSFLLTDDNEATSHTMSTAEVLLNMESPSDILDEKQIFSTSEMLPDSDPAPAVTLPNYLFPASEPDALNRAGDTSDQEGHSLEEKASREESAKKTGKSKKRIRKTKGNRSTSPVTDPSIPIRKKSKDGKGSTIYLWEFLLALLQDRNTCPKYIKWTQREKGIFKLVDSKAVSKLWGKQKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKEMPKDLVVIE Predict reactive species |
| Full Name | E74-like factor 4 (ets domain transcription factor) |
| Calculated Molecular Weight | 663 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa, 90~100 kDa |
| GenBank Accession Number | BC017194 |
| Gene Symbol | ELF4 |
| Gene ID (NCBI) | 2000 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99607 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ELF4 (E74-like ETS transcription factor 4), also widely known as MEF (Myeloid Elf-1-like factor), is a protein-coding gene in humans located on the X chromosome. It encodes a sequence-specific DNA-binding transcription factor that belongs to the evolutionary conserved ETS family. ELF4 is abundantly expressed in normal human hematopoietic tissues. It modulates the cell cycle, ensuring a balanced transition of hematopoietic stem cells and endothelial cells between a resting (quiescent) phase and active cell division (PMID: 40083328). IP analysis detected bands at 71 kDa, while the 90~100 kDa band could be post-translational modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ELF4 antibody 16020-1-AP | Download protocol |
| WB protocol for ELF4 antibody 16020-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
