Tested Applications
| Positive WB detected in | HeLa cells, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13946-1-AP targets EMB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5031 Product name: Recombinant human EMB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-253 aa of BC059398 Sequence: MRALPGLLEARARTPRLLLLQCLLAAARPSSADGSAPDSPFTSPPLREEIMANNFSLESHNISLTEHSSMPVEKNITLERPSNVNLTCQFTTSGDLNAVNVTWKKDGEQLENNYLVSATGSTLYTQYRFTIINSKQMGSYSCFFREEKEQRGTFNFKVPELHGKNKPLISYVGDSTVLTCKCQNCFPLNWTWYSSNGSVKVPVGVQMNKYVINGTYANETKLKITQLLEEDGESYWCRALFQLGESEEHIELV Predict reactive species |
| Full Name | embigin homolog (mouse) |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC059398 |
| Gene Symbol | EMB |
| Gene ID (NCBI) | 133418 |
| RRID | AB_2877993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PCB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycosylated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EMB antibody 13946-1-AP | Download protocol |
| WB protocol for EMB antibody 13946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (10-04-2021) | calculated size 37 kd. This antibody recognized major bands at 65, 55 kd, and a very weak band ~45 kd. All of them were PNGaseF insensitive, suggesting they were not glycosylated form of this protein.
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