Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11965-1-AP targets EMG1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2575 Product name: Recombinant human EMG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC055314 Sequence: MAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSDVRELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI Predict reactive species |
| Full Name | EMG1 nucleolar protein homolog (S. cerevisiae) |
| Calculated Molecular Weight | 244 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC055314 |
| Gene Symbol | EMG1 |
| Gene ID (NCBI) | 10436 |
| RRID | AB_874093 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92979 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EMG1, also named as NEP1, is a highly conserved protein initially identified as "Essential for Mitotic Growth" in yeast. EMG1 is required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. EMG1 is the methyltransferase in the biosynthesis of m1acp3-Psi in eukaryotic 18S rRNAs. It has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity. A mutation in human EMG1 gene causes Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EMG1 antibody 11965-1-AP | Download protocol |
| IP protocol for EMG1 antibody 11965-1-AP | Download protocol |
| WB protocol for EMG1 antibody 11965-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EMG1 Polyclonal antibody (Cat# 11965-1-AP) has 6 citations published in Nature Communications, Cell Reports, Molecular & Cellular Proteomics, International Journal of Molecular Sciences, and Biochimica et Biophysica Acta (Molecular Cell Research). Research fields span proteomics and drug target discovery, rRNA methylation and ribosome biogenesis, neurodevelopmental protein enrichment, goat oviduct biomarker profiling, m6A-mediated bladder cancer regulation, and the ribosomopathy Bowen-Conradi syndrome.
| Species | Application | Title |
|---|---|---|
Nat Commun Solvent-induced partial cellular fixation decodes proteome-wide drug targets and downstream pathways in living cells | ||
Mol Cell Proteomics Nucleolar enrichment of brain proteins with critical roles in human neurodevelopment. | ||
Int J Mol Sci Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods | ||
Biochim Biophys Acta Mol Cell Res FTO-mediated m6A demethylation inhibits bladder cancer progression via decreasing EMG1 and reducing ribosome biosynthesis. | ||
Biochim Biophys Acta Growth arrest in the ribosomopathy, Bowen-Conradi syndrome, is due to dramatically reduced cell proliferation and a defect in mitotic progression. |
Reviews
What customers say
The only available review for this product is from a researcher who used it for Western Blot (lot 00046181) and gave it a 5-star rating, noting that the antibody "seems specific." The review was posted in September 2025 and includes an image of the WB result. Overall, the limited feedback suggests satisfactory specificity for Western Blot applications, though more reviews would be needed to draw broader conclusions about performance across different conditions or applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nathalie (Verified Customer) (09-26-2025) | It seems specific
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