ENPP4 Polyclonal antibody

ENPP4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17305-1-AP

Synonyms

E NPP 4, ENPP4, KIAA0879



Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

17305-1-AP targets ENPP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENPP4 fusion protein Ag11192
Full Name ectonucleotide pyrophosphatase/phosphodiesterase 4 (putative function)
Calculated Molecular Weight 453 aa, 52 kDa
Observed Molecular Weight 52-60 kDa
GenBank Accession NumberBC018054
Gene Symbol ENPP4
Gene ID (NCBI) 22875
RRIDAB_2099780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENPP4, also named as KIAA0879 or NPP4, is a 453 amino acid protein, which belongs to the nucleotide pyrophosphatase/phosphodiesterase family. ENPP4 localizes in the cell membrane and hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. ENPP4 is expressed on the surface of vascular endothelia.

Protocols

Product Specific Protocols
WB protocol for ENPP4 antibody 17305-1-APDownload protocol
IHC protocol for ENPP4 antibody 17305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Blood

NPP4 is a procoagulant enzyme on the surface of vascular endothelium.

Authors - Albright Ronald A RA