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ENPP6 Polyclonal antibody

ENPP6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12643-1-AP

Synonyms

E NPP 6, ENPP6

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ENPP6 Polyclonal antibody (12643-1-AP)

    WB analysis of human brain using 12643-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12643-1-AP (ENPP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ENPP6 Polyclonal antibody (12643-1-AP)

    WB analysis of HEK-293 using 12643-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12643-1-AP (ENPP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ENPP6 Polyclonal antibody (12643-1-AP)

    IHC staining of mouse brain using 12643-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12643-1-AP (ENPP6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ENPP6 Polyclonal antibody (12643-1-AP)

    IHC staining of mouse brain using 12643-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12643-1-AP (ENPP6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12643-1-AP targets ENPP6 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENPP6 fusion protein Ag3339
Full Name ectonucleotide pyrophosphatase/phosphodiesterase 6
Calculated Molecular Weight 440 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC035035
Gene Symbol ENPP6
Gene ID (NCBI) 133121
RRIDAB_2099784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ENPP6 antibody 12643-1-APDownload protocol
IHC protocol for ENPP6 antibody 12643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols