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ARTS1 Polyclonal antibody

ARTS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13821-1-AP

Synonyms

A LAP, ALAP, Aminopeptidase PILS, APPILS, ARTS 1, ARTS1, ERAAP, ERAAP1, ERAP1, KIAA0525, PILS AP, PILSAP, Puromycin insensitive leucyl specific aminopeptidase

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ARTS1 Polyclonal antibody (13821-1-AP)

    WB analysis of K-562 using 13821-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13821-1-AP (ARTS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using ARTS1 Polyclonal antibody (13821-1-AP)

    IP experiment of Jurkat using 13821-1-AP

    IP result of anti-ARTS1 (IP:13821-1-AP, 4ug; Detection:13821-1-AP 1:600) with Jurkat cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ARTS1 Polyclonal antibody (13821-1-AP)

    IHC staining of human ovary tumor using 13821-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13821-1-AP (ARTS1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ARTS1 Polyclonal antibody (13821-1-AP)

    IHC staining of human ovary tumor using 13821-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13821-1-AP (ARTS1 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13821-1-AP targets ARTS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARTS1 fusion protein Ag4918
Full Name endoplasmic reticulum aminopeptidase 1
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 106 kDa
GenBank Accession NumberBC030775
Gene Symbol ERAP1
Gene ID (NCBI) 51752
RRIDAB_2293531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARTS1 is an endoplasmic reticulum-associated aminopeptidase (also known as ERAP1). ERAP1 is induced by interferon-gama and can degrade peptides 9-15 residues long(PMID:18987748).ARTS1 is a 100 kDa, monomeric, soluble zinc aminopeptidase. It can be glycosylated to 120KDa and form homodimers and heterodimers(PMID:15908954).

Protocols

Product Specific Protocols
WB protocol for ARTS1 antibody 13821-1-APDownload protocol
IHC protocol for ARTS1 antibody 13821-1-APDownload protocol
IP protocol for ARTS1 antibody 13821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

ERAP1-ERAP2 Dimerization Increases Peptide-Trimming Efficiency.

Authors - Irini Evnouchidou
View Article

Autoimmunity

Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of ankylosing spondylitis reduce HLA-B27 mediated presentation of multiple antigens.

Authors - Seregin Sergey S SS
View Article