ERLIN1 Polyclonal antibody

Name: ERLIN1 Polyclonal antibody
Brand: Proteintech
SKU: 17311-1-AP

ERLIN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17311-1-AP

Synonyms

C10orf69, ER lipid raft associated 1, Erlin 1, ERLIN1, KE04, KEO4, Protein KE04, SPFH1

Validation Data Gallery

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AllWBIPIHCIF

WB analysis using 17311-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17311-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 17311-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human ovary cancer using 17311-1-AP
human ovary cancer were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat pancreas using 17311-1-AP
rat pancreas tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 17311-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 17311-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 17311-1-AP
IP result of anti-ERLIN1 (IP:17311-1-AP, 3ug; Detection:17311-1-AP 1:1000) with mouse heart tissue lysate 4000ug.
IHC staining of mouse liver using 17311-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17311-1-AP (ERLIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 17311-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17311-1-AP (ERLIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17311-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17311-1-AP (ERLIN1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 17311-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17311-1-AP (ERLIN1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 17311-1-AP
Immunofluorescent analysis of HeLa cells using 17311-1-AP (ERLIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 17311-1-AP

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Tested Applications

Positive WB detected in	HL-60 cells, COLO 320 cells, human kidney tissue, human ovary cancer cells, rat pancreas tissue, human brain tissue, mouse liver tissue, U-251 cells, U-397 cells
Positive IP detected in	mouse heart tissue
Positive IHC detected in	mouse liver tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 1 publications below

Product Information

17311-1-AP targets ERLIN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ERLIN1 fusion protein Ag11279
Full Name	ER lipid raft associated 1
Calculated Molecular Weight	346 aa, 39 kDa
Observed Molecular Weight	39 kDa
GenBank Accession Number	BC031791
Gene Symbol	ERLIN1
Gene ID (NCBI)	10613
RRID	AB_2098590
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ERLIN1 antibody 17311-1-AP	Download protocol
IHC protocol for ERLIN1 antibody 17311-1-AP	Download protocol
IF protocol for ERLIN1 antibody 17311-1-AP	Download protocol
IP protocol for ERLIN1 antibody 17311-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation. Authors - Fabien Llambi View Article
human	WB	Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex Authors - Michael L van de Weijer View Article
human	WB	Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex. Authors - Michael L van de Weijer View Article
human	WB	Elife RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells. Authors - Kirsteen M Tullett View Article
human	WB	PLoS Genet Disruption of the ERLIN-TM6SF2-APOB complex destabilizes APOB and contributes to non-alcoholic fatty liver disease. Authors - Bo-Tao Li View Article
human	IHC,WB	Front Mol Biosci Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients Authors - Liping Shi View Article

ERLIN1 Polyclonal antibody

ERLIN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 17311-1-AP

WB analysis of mouse liver using 17311-1-AP

WB analysis of human kidney using 17311-1-AP

WB analysis of human ovary cancer using 17311-1-AP

WB analysis of rat pancreas using 17311-1-AP

WB analysis of human brain using 17311-1-AP

WB analysis of COLO 320 using 17311-1-AP

IP experiment of mouse heart using 17311-1-AP

IHC staining of mouse liver using 17311-1-AP

IHC staining of mouse liver using 17311-1-AP

IHC staining of human placenta using 17311-1-AP

IHC staining of human placenta using 17311-1-AP

IF Staining of HeLa using 17311-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells.

Disruption of the ERLIN-TM6SF2-APOB complex destabilizes APOB and contributes to non-alcoholic fatty liver disease.

Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients