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ESX1 Polyclonal antibody

ESX1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 14657-1-AP

Synonyms

ESX homeobox 1, ESX1, ESX1L, ESX1R, ESXR1, Homeobox protein ESX1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ESX1 Polyclonal antibody (14657-1-AP)

    WB analysis of mouse brain using 14657-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14657-1-AP (ESX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ESX1 Polyclonal antibody (14657-1-AP)

    WB analysis of mouse brain using 14657-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14657-1-AP (ESX1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ESX1 Polyclonal antibody (14657-1-AP)

    IP experiment of mouse brain using 14657-1-AP

    IP result of anti-ESX1 (IP:14657-1-AP, 4ug; Detection:14657-1-AP 1:500) with mouse brain tissue lysate 3440ug.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14657-1-AP targets ESX1 in WB, IP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ESX1 fusion protein Ag6269
Full Name ESX homeobox 1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC053599
Gene Symbol ESX1
Gene ID (NCBI) 80712
RRIDAB_10694138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESX1, also named as ESX1L and ESX1R , is an X-linked homeobox gene primarily expressed in the placenta and testis, with physiological functions in placenta/fetus development and spermatogenesis. It has a substantively different C-terminal region subject to strong purifying selection. ESX1 can be cleaved into 2 part: ESXR1-N(45kd) and ESXR1-C(20kd). ESXR1-N acts as a transcriptional repressor. ESXR1-C has the ability to inhibit cyclin turnover.

Protocols

Product Specific Protocols
WB protocol for ESX1 antibody 14657-1-APDownload protocol
IP protocol for ESX1 antibody 14657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols