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  • KD/KO Validated

EXO1 Polyclonal antibody

EXO1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16253-1-AP

Synonyms

EXO1, EXOI, exonuclease 1, Exonuclease I, HEX1, hExo1, hExoI



Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, HeLa cells, Raji cells
Positive IP detected inmouse thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16253-1-AP targets EXO1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXO1 fusion protein Ag9253
Full Name exonuclease 1
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 115-120 kDa
GenBank Accession NumberBC007491
Gene Symbol EXO1
Gene ID (NCBI) 9156
RRIDAB_2278140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.

Protocols

Product Specific Protocols
WB protocol for EXO1 antibody 16253-1-APDownload protocol
IP protocol for EXO1 antibody 16253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway.

Authors - Margaret Tammaro
humanWB

Nucleic Acids Res

PCNA promotes processive DNA end resection by Exo1.

Authors - Chen Xiaoqing X
  • KD Validated
humanWB

Elife

Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations.

Authors - Birong Shen
  • KD Validated
humanWB

PLoS Genet

EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair.

Authors - Yuehan Wu
ratWB

J Cardiovasc Transl Res

Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.

Authors - Xinliang Zou
humanWB

Front Genet

Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA.

Authors - Christopher B Nelson
  • KD Validated