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EXOC6B Polyclonal antibody

EXOC6B Polyclonal Antibody for IF, IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55440-1-AP

Synonyms

EXOC6B, exocyst complex component 6B, KIAA0919, SEC15 like protein 2, SEC15B, SEC15L2

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293 cells using EXOC6B Polyclonal antibody (55440-1-AP)

    IP experiment of HEK-293 using 55440-1-AP

    IP result of anti-EXOC6B (IP:55440-1-AP, 4ug; Detection:55440-1-AP 1:500) with HEK-293 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using EXOC6B Polyclonal antibody (55440-1-AP)

    IHC staining of human liver using 55440-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55440-1-AP (EXOC6B antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using EXOC6B Polyclonal antibody (55440-1-AP)

    IHC staining of human liver using 55440-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55440-1-AP (EXOC6B antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EXOC6B Polyclonal antibody (55440-1-AP)

    IF Staining of HEK-293 using 55440-1-AP

    Immunofluorescent analysis of HEK-293 cells using 55440-1-AP (EXOC6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55440-1-AP targets EXOC6B in IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name exocyst complex component 6B
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberNM_015189
Gene Symbol EXOC6B
Gene ID (NCBI) 23233
RRIDAB_2881333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for EXOC6B antibody 55440-1-APDownload protocol
IF protocol for EXOC6B antibody 55440-1-APDownload protocol
IP protocol for EXOC6B antibody 55440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols