Filter: AllWBIHC

WB analysis of HeLa using 20911-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20911-1-AP (FAM185A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20911-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 20911-1-AP (FAM185A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20911-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20911-1-AP (FAM185A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human hepatocirrhosis using 20911-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20911-1-AP (FAM185A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human hepatocirrhosis using 20911-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20911-1-AP (FAM185A antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 20911-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 20911-1-AP (FAM185A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 20911-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 20911-1-AP (FAM185A antibody) at dilution of 1:50 (under 40x lens).

FAM185A Polyclonal antibody

Name: FAM185A Polyclonal antibody
Brand: Proteintech
SKU: 20911-1-AP

Cat No. 20911-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

FAM185A, Protein FAM185A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	HeLa cells, HEK-293 cells, MCF-7 cells
Positive IHC detected in	human hepatocirrhosis tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

20911-1-AP targets FAM185A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15041 Product name: Recombinant human FAM185A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-144 aa of BC029175 Sequence: MLAPCSGWELGCFRLCLRQVRLWAGAGRWACWACQARPYSSGGSERWPGSETEVPPPGPARRTLKEWTLQVSPFGRLRARLPCHLAVRPLDPLTYPDGDRVLVAVCGVEGGVRGLDGLQVKYDEDLEEMAIVSDTIHPQASVEV Predict reactive species
Full Name	family with sequence similarity 185, member A
Calculated Molecular Weight	393 aa, 42 kDa
Observed Molecular Weight	30 kDa, 42 kDa
GenBank Accession Number	BC029175
Gene Symbol	FAM185A
Gene ID (NCBI)	222234
RRID	AB_10732807
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N0U4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.