FANCG Polyclonal antibody

FANCG Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 10215-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

XRCC9, Protein FACG, Fanconi anemia group G protein, FAG, DNA repair protein XRCC9

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIF/ICC

WB analysis of Jurkat using 10215-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse thymus using 10215-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of K-562 using 10215-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 10215-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 10215-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse testis using 10215-1-AP
IP result of anti-FANCG (IP:10215-1-AP, 4ug; Detection:10215-1-AP 1:300) with mouse testis tissue lysate 4000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 10215-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCG antibody (10215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, mouse testis tissue, K-562 cells, HeLa cells, mouse thymus tissue
Positive IP detected in	mouse testis tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10215-1-AP targets FANCG in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0249 Product name: Recombinant human FANCG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 125-348 aa of BC000032 Sequence: VLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKH Predict reactive species
Full Name	Fanconi anemia, complementation group G
Calculated Molecular Weight	69 kDa
Observed Molecular Weight	65-68 kDa
GenBank Accession Number	BC000032
Gene Symbol	FANCG
Gene ID (NCBI)	2189
RRID	AB_2231541
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15287
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546).

Protocols

Product Specific Protocols
IF protocol for FANCG antibody 10215-1-AP	Download protocol
IP protocol for FANCG antibody 10215-1-AP	Download protocol
WB protocol for FANCG antibody 10215-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair Authors - Dinh Duc Nguyen View Article
human	IF	J Cancer Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma. Authors - Yi Liu View Article
human	WB	Clin Transl Oncol Therapeutic benefits of niraparib tosylate as radio sensitizer in esophageal squamous cell carcinoma: an in vivo and in vitro preclinical study. Authors - Yuzhong Cui View Article
		Nat Commun Histone-methyltransferase KMT2D deficiency impairs the Fanconi anemia/BRCA pathway upon glycolytic inhibition in squamous cell carcinoma Authors - Wei Liu View Article