Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16436-1-AP targets FARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9750 Product name: Recombinant human FARS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 109-451 aa of BC021112 Sequence: LFSVYDNLSPVVTTWQNFDSLLIPADHPSRKKGDNYYLNRTHMLRAHTSAHQWDLLHAGLDAFLVVGDVYRRDQIDSQHYPIFHQLEAVRLFSKHELFAGIKDGESLQLFEQSSRSAHKQETHTMEAVKLVEFDLKQTLTRLMAHLFGDELEIRWVDCYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQFCVSNINQKVKFQPLSKYPAVINDISFWLPSENYAENDFYDLVRTIGGDLVEKVDLIDKFVHPKTHKTSHCYRITYRHMERTLSQREVRHIHQALQEAAVQLLGVEGRF Predict reactive species |
| Full Name | phenylalanyl-tRNA synthetase 2, mitochondrial |
| Calculated Molecular Weight | 451 aa, 52 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC021112 |
| Gene Symbol | FARS2 |
| Gene ID (NCBI) | 10667 |
| RRID | AB_2102499 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95363 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FARS2, a nuclear gene located on chromosome 6 (6p25.1), encodes the mitochondrial phenylalanyl-tRNA synthetase (mtPheRS), which transfers phenylalanine (Phe) to its cognate tRNA in mitochondria (PMID: 32774346).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FARS2 antibody 16436-1-AP | Download protocol |
| IHC protocol for FARS2 antibody 16436-1-AP | Download protocol |
| WB protocol for FARS2 antibody 16436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Mol Genet Metab Clinical and molecular characterization of novel FARS2 variants causing neonatal mitochondrial disease | ||
Biochim Biophys Acta Mutation of the human mitochondrial phenylalanine-tRNA synthetase causes infantile-onset epilepsy and cytochrome c oxidase deficiency. | ||
Eur J Hum Genet Novel FARS2 variants in patients with early onset encephalopathy with or without epilepsy associated with long survival. | ||
Nat Commun Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ. |
