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FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 11394-1-AP

Synonyms

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, HCG1989313, isoform CRA_b, KIAA1863

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using FBF1 Polyclonal antibody (11394-1-AP)

    WB analysis of mouse brain using 11394-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11394-1-AP (FBF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using FBF1 Polyclonal antibody (11394-1-AP)

    IP experiment of mouse brain using 11394-1-AP

    IP result of anti-FBF1 (IP:11394-1-AP, 4ug; Detection:11394-1-AP 1:400) with mouse brain tissue lysate 5000ug.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11394-1-AP targets FBF1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBF1 fusion protein Ag1954
Full Name Fas (TNFRSF6) binding factor 1
Calculated Molecular Weight1133 aa, 125 kDa
Observed Molecular Weight 69-70 kDa
GenBank Accession NumberBC012332
Gene Symbol FBF1
Gene ID (NCBI) 85302
RRIDAB_10638320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by the fragment (492-792aa) of FBF1. It recognizes isoform 1, 2, 3, 5 and 6.

Protocols

Product Specific Protocols
WB protocol for FBF1 antibody 11394-1-APDownload protocol
IP protocol for FBF1 antibody 11394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols