Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 16021-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 16021-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16021-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16021-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A375 using 16021-1-AP
IP result of anti-FBL (IP:16021-1-AP, 4ug; Detection:16021-1-AP 1:500) with A375 cells lysate 3600ug.
IHC staining of mouse brain using 16021-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16021-1-AP (FBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16021-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 16021-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 16021-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 16021-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FBL (16021-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

FBL Polyclonal antibody

Name: FBL Polyclonal antibody
Brand: Proteintech
SKU: 16021-1-AP
Rating: 5.00 (4 reviews)

FBL Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16021-1-AP

Publications(35)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, RIP, ELISA

Synonyms

EC:2.1.1.-, FIB, FIB1, fibrillarin, FLRN

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (35)
Protocols
Reviews (4)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A375 cells, mouse liver tissue, mosue kidney tissue
Positive IP detected in	A375 cells
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 22 publications below
IF	See 11 publications below
IP	See 1 publications below
RIP	See 4 publications below

Product Information

16021-1-AP targets FBL in WB, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8841 Product name: Recombinant human FBL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-321 aa of BC019260 Sequence: MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN Predict reactive species
Full Name	fibrillarin
Calculated Molecular Weight	321 aa, 34 kDa
Observed Molecular Weight	34 kDa
GenBank Accession Number	BC019260
Gene Symbol	FBL
Gene ID (NCBI)	2091
RRID	AB_2105788
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22087
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FBL antibody 16021-1-AP	Download protocol
IHC protocol for FBL antibody 16021-1-AP	Download protocol
IF protocol for FBL antibody 16021-1-AP	Download protocol
IP protocol for FBL antibody 16021-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Oxidative cyclization reagents reveal tryptophan cation-π interactions Authors - Xiao Xie View Article
human	WB,RIP,IF	Mol Cell lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing Authors - Yu-Meng Sun View Article
human	WB	Mol Cell LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice. Authors - Xin Wang KD Validated View Article
human	WB	Nucleic Acids Res GPATCH4 functions as a regulator of nucleolar R-loops in hepatocellular carcinoma cells Authors - Yi-Ming Zhao View Article
human	WB	Breast Cancer Res U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression Authors - Wenrong Zhang View Article
human	WB,IF	Cell Biol Toxicol SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells Authors - Jingyi Cui View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Tatyana (Verified Customer) (03-11-2023)	Very strong, specific nucleolar signal in ICC. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h RT incubation (no blocking step). Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human, U2OS cells
FH WEI (Verified Customer) (03-12-2020)	Good, strong band for WB and correct staining for cardiomyocytes. Applications: Western Blot, Immunofluorescence, Cell Tissue Type: Mouse Heart
FH Lauren (Verified Customer) (02-20-2020)	Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal. Applications: Immunofluorescence, Primary Antibody Dilution: 1:100 Cell Tissue Type: mIMCD3 FBL Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:16021-1-AP)
FH Baptiste (Verified Customer) (09-05-2019)	This antibody works well in western blot Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: human satelllite muscle cells FBL Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:16021-1-AP)