FBLN5 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IP|
|Species specificity||human, mouse, rat; other species not tested.|
|Positive WB detected in||mouse heart tissue, rat brain tissue, mouse placenta tissue|
|Positive IHC detected in||human lung cancer|
|Recommended dilution||WB: 1:200-1:2000|
IP = Immunoprecipitation
- IP result of anti-FBLN5 (12188-1-AP for IP and Detection) with mouse heart tissue.
- mouse heart tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP(FBLN5 Antibody) at dilution of 1:500
- Immunohistochemistry of paraffin-embedded human lung cancer using 12188-1-AP(FBLN5 Antibody) at Dilution 1:50 (under 10x lens)
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||FBLN5 fusion protein ag2859||Full name||fibulin 5|
|BC022280||Gene ID (NCBI)||10516|
|Gene symbol||FBLN5||Synonyms||ARMD3; DANCE; EVEC; FIBL-5; FLJ90059; UP50|
Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50kd or 66kd band in western blotting, the 66kd corresponding to fully glycosylated protein.