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fibrillin 2-Specific Polyclonal antibody

fibrillin 2-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20252-1-AP

Synonyms

CCA, DA9, FBN2, fibrillin 2, fibrillin 2-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    WB analysis of human brain using 20252-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    WB analysis of human brain using 20252-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    WB analysis of HepG2 using 20252-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    WB analysis of HepG2 using 20252-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    WB analysis of mouse lung using 20252-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    WB analysis of L02 using 20252-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    WB analysis of L02 using 20252-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    IHC staining of human placenta using 20252-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    IHC staining of human placenta using 20252-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    IHC staining of human placenta using 20252-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using fibrillin 2-Specific Polyclonal antibody (20252-1-AP)

    IHC staining of human placenta using 20252-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue, HepG2 cells, L02 cells, mouse lung tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20252-1-AP targets fibrillin 2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name fibrillin 2
Calculated Molecular Weight 315 kDa
Observed Molecular Weight 290 kDa, 160 kDa
GenBank Accession NumberNM_001999
Gene Symbol FBN2
Gene ID (NCBI) 2201
RRIDAB_11182265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBN2 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils which occur either in association with elastin or in elastin-free bundles. Fibrillin-2-containing microfibrils regulate the early process of elastic fiber assembly. Defects in FBN2 are the cause of congenital contractural arachnodactyly (CCA) which also known as Beals syndrome or distal arthrogryposis type 9 (DA9). This antibody is specific to FBN2. It recognizes both two isoforms ~300 kDa and 160 kDa.

Protocols

Product Specific Protocols
WB protocol for fibrillin 2-Specific antibody 20252-1-APDownload protocol
IHC protocol for fibrillin 2-Specific antibody 20252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Head Neck

High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas.

Authors - Sreenivasulu Chintala
View Article
mouseIHC

J Ovarian Res

hUMSC transplantation restores follicle development in ovary damaged mice via re-establish extracellular matrix (ECM) components

Authors - Yin Shuyuan
View Article