FBP17 Polyclonal antibody

FBP17 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27056-1-AP

Synonyms

FBP17, FNBP1, formin binding protein 1, Formin binding protein 17, hFBP17, KIAA0554



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:150-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27056-1-AP is FBP17 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBP17 fusion protein Ag25784
Full Name formin binding protein 1
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC101755
Gene Symbol FNBP1
Gene ID (NCBI) 23048
RRIDAB_2880734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBP17 (formin-binding protein 17) is a new dynamin-associating molecule consisting of several functional domains, including an N-terminal EFC domain and an SH3 domain at the C-terminus. FBP17 is involved in dynamin-mediated endocytosis and has been reported to be required for invadopodia formation and tumor cell invasion.

Protocols

Product Specific Protocols
WB protocol for FBP17 antibody 27056-1-APDownload protocol
IHC protocol for FBP17 antibody 27056-1-APDownload protocol
IP protocol for FBP17 antibody 27056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

María (Verified Customer) (02-08-2022)

Works fine for WB (1:1000), for IF some background, even after several washes with detergent

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela/RPE-1/MEF/NaTi