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  • Immunoprecipitation (IP) experiment of HCT 116 cells using FBXL8 Polyclonal antibody (16505-1-AP)

    IP experiment of HCT 116 using 16505-1-AP

    IP result of anti-FBXL8 (IP:16505-1-AP, 4ug; Detection:16505-1-AP 1:500) with HCT 116 cells lysate 1920 ug.

FBXL8 Polyclonal antibody

FBXL8 Polyclonal Antibody for IP, ELISA
Cat No. 16505-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, ELISA

Synonyms

FBL8, F-box and leucine-rich repeat protein 8, F-box protein FBL8, F-box/LRR-repeat protein 8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive IP detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16505-1-AP targets FBXL8 in IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXL8 fusion protein Ag9685 Predict reactive species
Full Name F-box and leucine-rich repeat protein 8
Calculated Molecular Weight 374 aa, 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC014414
Gene Symbol FBXL8
Gene ID (NCBI) 55336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXL8 is a conserved F-box protein that belongs to the ubiquitin ligase complex. It plays a significant role in various cellular processes, including tumorigenesis and metastasis. In colorectal cancer, FBXL8 promotes liver metastasis and stem-cell-like features by mediating ubiquitination and degradation of TP53. Additionally, FBXL8 has been shown to inhibit lymphoma growth and hematopoietic malignancies by targeting specific substrates for degradation. In the context of cardiac fibrosis, FBXL8 inhibits post-myocardial infarction cardiac fibrosis by regulating the ubiquitination of key proteins (PMID: 36855778, PMID: 33122824, PMID: 38615011).

Protocols

Product Specific Protocols
IP protocol for FBXL8 antibody 16505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols