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KD/KO Validated

FGD3 Polyclonal antibody

FGD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20347-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Zinc finger FYVE domain-containing protein 5, ZFYVE5, FYVE, RhoGEF and PH domain-containing protein 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHCIF/ICC

WB analysis using 20347-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of K-562 using 20347-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293T using 20347-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 20347-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20347-1-AP (FGD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 20347-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20347-1-AP (FGD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A549 using 20347-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 20347-1-AP (FGD3 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, K-562 cells, HEK-293T cells, HeLa cells, A549 cells
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20347-1-AP targets FGD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14155 Product name: Recombinant human FGD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 570-725 aa of BC032429 Sequence: KAENSRQSRVCRDCFLTQPVAPESTEKTPTADPQPSLLCGPLRLSESGETWSEVWAAIPMSDPQVLHLQGGSQDGRLPRTIPLPSCKLSVPDPEERLDSGHVWKLQWAKQSWYLSASSAELQQQWLETLSTAAHGDTAQDSPGALQLQVPMGAAAP Predict reactive species
Full Name	FYVE, RhoGEF and PH domain containing 3
Calculated Molecular Weight	725 aa, 79 kDa
Observed Molecular Weight	79 kDa
GenBank Accession Number	BC032429
Gene Symbol	FGD3
Gene ID (NCBI)	89846
RRID	AB_10693616
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5JSP0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for FGD3 antibody 20347-1-AP	Download protocol
IHC protocol for FGD3 antibody 20347-1-AP	Download protocol
WB protocol for FGD3 antibody 20347-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB,IHC	Oncogene FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression. Authors - Feng Guo KD Validated View Article
	WB	Mol Ther Oncol Dual CARM1-and IKZF3-targeting: A novel approach to multiple myeloma therapy synergy between CARM1 inhibition and IMiDs Authors - Wei Ni View Article
human	WB,IHC	Cancers (Basel) A Zinc Finger Protein-Based Prognostic Model in Lung Adenocarcinoma Identifies FGD3 as a Marker Associated with Lorlatinib Resistance. Authors - Jiayue Sun KD Validated View Article
human	WB	J Exp Clin Cancer Res FGD3 mediates lytic cell death, enhancing efficacy and immunogenicity of chemotherapy agents in breast cancer. Authors - Junyao Zhu KO Validated View Article