FGF1 Polyclonal antibody

FGF1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17400-1-AP

Synonyms

AFGF, ECGF, ECGF beta, ECGFA, ECGFB, FGF alpha, FGF1, FGFA, GLIO703, HBGF 1, HBGF1



Tested Applications

Positive WB detected inmouse brain tissue, mouse spinal cord tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human lung cancer tissue, mouse brain tissue, rat brain tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

17400-1-AP targets FGF1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGF1 fusion protein Ag11366
Full Name fibroblast growth factor 1 (acidic)
Calculated Molecular Weight 155 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC032697
Gene Symbol FGF1
Gene ID (NCBI) 2246
RRIDAB_2103758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FGF1 antibody 17400-1-APDownload protocol
IHC protocol for FGF1 antibody 17400-1-APDownload protocol
IF protocol for FGF1 antibody 17400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuropharmacology

Non-mitogenic fibroblast growth factor 1 protects against ischemic stroke by regulating microglia/macrophage polarization through Nrf2 and NF-κB pathways.

Authors - Confidence Dordoe
humanWB

Life Sci

A derivant of ginsenoside CK and its inhibitory effect on hepatocellular carcinoma.

Authors - Jingjing Zhang
pigWB

Physiol Rep

Sitagliptin therapy improves myocardial perfusion and arteriolar collateralization in chronically ischemic myocardium: A pilot study

Authors - Sharif A Sabe
mouseWB

J Cereb Blood Flow Metab

Saturated fatty acids stimulate cytokine production in tanycytes via the PP2Ac-dependent signaling pathway

Authors - Danyang Liu
mouseWB

Stem Cell Res Ther

Ectoderm-derived frontal bone mesenchymal stem cells promote traumatic brain injury recovery by alleviating neuroinflammation and glutamate excitotoxicity partially via FGF1.

Authors - Qiaozhen Qin