Tested Applications
| Positive WB detected in | HeLa cells, HuH-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17604-1-AP targets FIGNL1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11709 Product name: Recombinant human FIGNL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 326-674 aa of BC051867 Sequence: KSLGASRSRGILGKFVPPIPKQDGGEQNGGMQCKPYGAGPTEPAHPVDERLKNLEPKMIELIMNEIMDHGPPVNWEDIAGVEFAKATIKEIVVWPMLRPDIFTGLRGPPKGILLFGPPGTGKTLIGKCIASQSGATFFSISASSLTSKWVGEGEKMVRALFAVARCQQPAVIFIDEIDSLLSQRGDGEHESSRRIKTEFLVQLDGATTSSEDRILVVGATNRPQEIDEAARRRLVKRLYIPLPEASARKQIVINLMSKEQCCLSEEEIEQIVQQSDAFSGADMTQLCREASLGPIRSLQTADIATITPDQVRPIAYIDFENAFRTVRPSVSPKDLELYENWNKTFGCGK Predict reactive species |
| Full Name | fidgetin-like 1 |
| Calculated Molecular Weight | 674 aa, 74 kDa |
| Observed Molecular Weight | 66-71 kDa |
| GenBank Accession Number | BC051867 |
| Gene Symbol | FIGNL1 |
| Gene ID (NCBI) | 63979 |
| RRID | AB_2104675 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PIW4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fidgetin-like 1 (FIGNL1), a subfamily member of ATPases, is associated with diverse cellular activities (AAA proteins). FIGNL1 is involved in DNA repair.FIGNL1 plays a crucial role in the occurrence and development of malignant tumors. FIGNL1 participates in the prognosis, promoter methylation, and immune infiltrates in KIRP, LGG, and LIHC (PMID: 35232348).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FIGNL1 antibody 17604-1-AP | Download protocol |
| IP protocol for FIGNL1 antibody 17604-1-AP | Download protocol |
| WB protocol for FIGNL1 antibody 17604-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell Rep C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair
| ||
Curr Gene Ther FIGNL1 Promotes Hepatocellular Carcinoma Formation via Remodeling ECM-receptor Interaction Pathway Mediated by HMMR | ||
Nat Commun FLIP(C1orf112)-FIGNL1 complex regulates RAD51 chromatin association to promote viability after replication stress | ||
Nat Commun FIGNL1-FIRRM is essential for meiotic recombination and prevents DNA damage-independent RAD51 and DMC1 loading |
