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WB analysis of HeLa using 11755-1-AP
WB result of FKBP3 antibody (11755-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP3 transfected HeLa cells.
WB analysis of PC-3 using 11755-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11755-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11755-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 11755-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 11755-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11755-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11755-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11755-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 11755-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 11755-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11755-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FKBP3 antibody (11755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HepG2 using 11755-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FKBP3 antibody (11755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

FKBP3 Polyclonal antibody

FKBP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11755-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FKBP25, FKBP 3, FKBP 25, FK506-binding protein 3, EC:5.2.1.8

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	PC-3 cells, HeLa cells, A431 cells
Positive IHC detected in	mouse brain tissue, human lung cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below

Product Information

11755-1-AP targets FKBP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2351 Product name: Recombinant human FKBP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC020809 Sequence: MAAAVPQRAWTVEQLRSEQLPKKDIIKFLQEHGSDSFLAEHKLLGNIKNVAKTANKDHLVTAYNHLFETKRFKGTESISKVSEQVKNVKLNEDKPKETKSEETLDEGPPKYTKSVLKKGDKTNFPKKGDVVHCWYTGTLQDGTVFDTNIQTSAKKKKNAKPLSFKVGVGKVIRGWDEALLTMSKGEKARLEIEPEWAYGKKGQPDAKIPPNAKLTFEVELVDID Predict reactive species
Full Name	FK506 binding protein 3, 25kDa
Calculated Molecular Weight	224 aa, 25 kDa
Observed Molecular Weight	25-30 kDa
GenBank Accession Number	BC020809
Gene Symbol	FKBP3
Gene ID (NCBI)	2287
RRID	AB_2102983
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q00688
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FKBP3 antibody 11755-1-AP	Download protocol
IHC protocol for FKBP3 antibody 11755-1-AP	Download protocol
IF protocol for FKBP3 antibody 11755-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cell Mol Med FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling Authors - Xiaojing Xing KD Validated View Article
human	IHC	BMC Cancer Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma. Authors - Yang Zhai View Article