Tested Applications
| Positive WB detected in | Raji cells, Jurkat cells, HeLa cells |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, Saos-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:500 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10395-1-AP targets FMNL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0594 Product name: Recombinant human FMNL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 39-351 aa of BC001710 Sequence: ELDMSDFEEQFKTKSQGPSLDLSALKSKAAQKAPSKATLIEANRAKNLAITLRKGNLGAERICQAIEAYDLQALGLDFLELLMRFLPTEYERSLITRFEREQRPMEELSEEDRFMLCFSRIPRLPERMTTLTFLGNFPDTAQLLMPQLNAIIAASMSIKSSDKLRQILEIVLAFGNYMNSSKRGAAYGFRLQSLDALLEMKSTDRKQTLLHYLVKVIAEKYPQLTGFHSDLHFLDKAGSVSLDSVLADVRSLQRGLELTQREFVRQDDCMVLKEFLRANSPTMDKLLADSKTAQEAFESVVEYFGENPKTTSP Predict reactive species |
| Full Name | formin-like 1 |
| Calculated Molecular Weight | 160 kDa |
| Observed Molecular Weight | 150-160 kDa |
| GenBank Accession Number | BC001710 |
| Gene Symbol | FMNL1 |
| Gene ID (NCBI) | 752 |
| RRID | AB_10895969 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95466 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. The MW of FMNL1 is about 150-160 kDa. (PMID: 16494944 )
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FMNL1 antibody 10395-1-AP | Download protocol |
| IHC protocol for FMNL1 antibody 10395-1-AP | Download protocol |
| WB protocol for FMNL1 antibody 10395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
