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WB analysis of HEK-293 using 22173-1-AP
WB result of FOXP3 antibody (22173-1-AP, 1:500) with si-Control and si-FOXP3 transfected HEK293 cells.
WB analysis of HEK-293 using 22173-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 22173-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22173-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22173-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22173-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 22173-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 22173-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22173-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 22173-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22173-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Featured Product
KD/KO Validated

FOXP3 Polyclonal antibody

Name: FOXP3 Polyclonal antibody
Brand: Proteintech
SKU: 22173-1-AP

FOXP3 Polyclonal Antibody for WB, ELISA

Cat No. 22173-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

HEK-293 cells, HEK-293

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

22173-1-AP targets FOXP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17827 Product name: Recombinant human FOXP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 230-431 aa of BC113401 Sequence: AQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDYFKFHNMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKRSQRPSRCSNPTPGP Predict reactive species
Full Name	forkhead box P3
Calculated Molecular Weight	431 aa, 47 kDa
Observed Molecular Weight	47-50 kDa
GenBank Accession Number	BC113401
Gene Symbol	FOXP3
Gene ID (NCBI)	50943
RRID	AB_2879017
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZS1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. FOXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Protocols

Product Specific Protocols
WB protocol for FOXP3 antibody 22173-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Exp Ther Med Poly(ADP-ribose) polymerase-1 inhibitor ameliorates dextran sulfate sodium-induced colitis in mice by regulating the balance of Th17/Treg cells and inhibiting the NF-κB signaling pathway. Authors - Shuai Peng View Article
human	IHC	Oral Dis Influence of PD-1/PD-L1 on immune micro-environment in oral leukoplakia and oral squamous cell carcinomas Authors - Qiong Wang View Article