Tested Applications
| Positive WB detected in | human plasma tissue, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
13086-1-AP targets FREM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3617 Product name: Recombinant human FREM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 412-710 aa of BC031064 Sequence: SSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSPRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYGCSRKL Predict reactive species |
| Full Name | FRAS1 related extracellular matrix 1 |
| Calculated Molecular Weight | 2179aa, 244 kDa, 149.3 kDa |
| Observed Molecular Weight | 40-43 kDa, 70-80 kDa |
| GenBank Accession Number | BC031064 |
| Gene Symbol | FREM1 |
| Gene ID (NCBI) | 158326 |
| RRID | AB_2877915 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5H8C1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FREM1, C9orf143, C9orf145, C9orf154 and QBRICK, is an extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. This antibody recognizes isoform 1 (244 kDa), isform 2 (80 kDa) and isoform 3 (43 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FREM1 antibody 13086-1-AP | Download protocol |
| WB protocol for FREM1 antibody 13086-1-AP | Download protocol |
| IF protocol for FREM1 antibody 13086-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) TILRR (FREM1 isoform 2) is a prognostic biomarker correlated with immune infiltration in breast cancer. | ||
Cancer Med Elevated expression of FREM1 in breast cancer indicates favorable prognosis and high-level immune infiltration status. |
