FSD2 Polyclonal antibody

FSD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25609-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

FSD2, RP11 127F21, SPRYD1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHC

WB analysis of mouse heart using 25609-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 25609-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat heart using 25609-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 25609-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 25609-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue, rat heart tissue
Positive IHC detected in	mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25609-1-AP targets FSD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22229 Product name: Recombinant human FSD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC130569 Sequence: MEEESGEELGLDRSTPKDFHFYHMDLYDSEDRLHLFPEENTRMRKVVQAEMANESRGAGDGKAQRDLQEEVDELVHLYGLEDDHELGDEFVDENIPRTGVSEYPPYMMKRRDPAREQRDWRLSGEAAEAEDLGFGGWGSAGQCQDLREAYRYTHGRASEEYECYVIPEEEDEEEAADVFCVTCKTPIRAFQKVFDEHKEHEVIPLNEALESAKDEIHKNMYKLEKQIIEMENFANHLEEVFITVEENFGKQEQNFESHYNEILETLAQKYEEKIQALGEKKKEKLEALYGQLVSCGENLDTCKELMETIEEMCHEEKVDFIKDAVAMADRLGKFLKTKTDVEISAQPEFE Predict reactive species
Full Name	fibronectin type III and SPRY domain containing 2
Calculated Molecular Weight	749 aa, 85 kDa
Observed Molecular Weight	95-105 kDa
GenBank Accession Number	BC130569
Gene Symbol	FSD2
Gene ID (NCBI)	123722
RRID	AB_2880159
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	A1L4K1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.