FUS Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | IF, IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | HEK-293 cells, HeLa cells, HL-60 cells, human kidney tissue, Jurkat cells, K-562 cells, SMMC-7721 cells |
| Positive IHC detected in | human ovary tumor |
| Positive IF detected in | HepG2 cells |
| ApplicationNote: | for IHC, use 60160-1-Ig (mAB) |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:50 |
IP = Immunoprecipitation
IP result of anti-FUS (11570-1-AP for IP and Detection) with K562 cell.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11570-1-AP(FUS Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human ovary tumor using 11570-1-AP(FUS Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human ovary tumor using 11570-1-AP(FUS Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using FUS antibody 11570-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | FUS fusion protein ag2150 | Full name | fusion (involved in t(12;16) in malignant liposarcoma) |
|
Calculated molecular weight |
75kd |
Observed molecular
weight |
73kd |
|
GenBank accession number |
BC026062 | Gene ID (NCBI) | 2521 |
| Gene symbol | FUS | Synonyms | CHOP; FUS-CHOP; FUS1; TLS; TLS/CHOP; hnRNP-P2 |
FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U). This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. |
