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Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human testis tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
17175-1-AP targets FUT11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10815 Product name: Recombinant human FUT11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 143-492 aa of BC036037 Sequence: ALLHEESPLNNFLLSHGPGIRLFNLTSTFSRHSDYPLSLQWLPGTAYLRRPVPPPMERAEWRRRGYAPLLYLQSHCDVPADRDRYVRELMRHIPVDSYGKCLQNRELPTARLQDTATATTEDPELLAFLSRYKFHLALENAICNDYMTEKLWRPMHLGAVPVYRGSPSVRDWMPNNHSVILIDDFESPQKLAEFIDFLDKNDEEYMKYLAYKQPGGITNQFLLDSLKHREWGVNDPLLPNYLNGFECFVCDYELARLDAEKAHAASPGDSPVFEPHIAQPSHMDCPVPTPGFGNVEEIPENDSWKEMWLQDYWQGLDQGEALTAMIHNNETEQTKFWDYLHEIFMKRQHL Predict reactive species |
| Full Name | fucosyltransferase 11 (alpha (1,3) fucosyltransferase) |
| Calculated Molecular Weight | 492 aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC036037 |
| Gene Symbol | FUT11 |
| Gene ID (NCBI) | 170384 |
| RRID | AB_2231850 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q495W5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FUT11 antibody 17175-1-AP | Download protocol |
| WB protocol for FUT11 antibody 17175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Dry and wet experiments reveal the significant role of FUT11 in clear cell renal cell carcinoma | ||
Cell Biol Int FUT11 is a target gene of HIF1α that promotes the progression of hepatocellular carcinoma. | ||
Heliyon Knockdown of FUT11 inhibits the progression of gastric cancer via the PI3K/AKT pathway | ||
Discov Oncol FUT11 expression in gastric cancer: its prognostic significance and role in immune regulation | ||
Nat Chem Biol FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains | ||
BMC Cancer Fucosyltransferase 11 restrains ferroptosis via upregulation GPX4 expression in gastric cancer |
