Tested Applications
| Positive ELISA detected in | peptide |
Recommended dilution
| Application | Dilution |
|---|---|
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24492-1-AP targets GA repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | GA repeat |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2879571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "GA repeat" sequence. This antibody detects the GAGAGAGAGA peptide with dilution 1:32,000 in Elisa.
Publications
What published studies show
The GA repeat Polyclonal antibody (Cat# 24492-1-AP) has 20 citations spanning journals including Cell, Nature Neuroscience, Neuron, Journal of Clinical Investigation, EMBO Molecular Medicine, Cell Reports, Molecular Neurobiology, Experimental Neurology, Stem Cells, Journal of Biological Chemistry, Journal of Cell Science, Human Molecular Genetics, Life Science Alliance, and Neuropathology & Applied Neurobiology. Citations focus on C9orf72-associated ALS/FTD research, particularly dipeptide repeat protein pathology and neurodegeneration.
| Species | Application | Title |
|---|---|---|
Nat Neurosci Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology. | ||
Neuron Disruption of nuclear speckle integrity dysregulates RNA splicing in C9ORF72-FTD/ALS | ||
J Clin Invest CRISPR-Cas13d targeting suppresses repeat-associated non-AUG translation of C9orf72 hexanucleotide repeat RNA | ||
EMBO Mol Med Active poly-GA vaccination prevents microglia activation and motor deficits in a C9orf72 mouse model. | ||
Cell Rep Altered MICOS Morphology and Mitochondrial Ion Homeostasis Contribute to Poly(GR) Toxicity Associated with C9-ALS/FTD. |
