Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10408-1-AP targets GAD1 in WB, IHC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0666 Product name: Recombinant human GAD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC002815 Sequence: MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMPSDMRECWLLR Predict reactive species |
| Full Name | glutamate decarboxylase 1 (brain, 67kDa) |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 67 kDa, 55 kDa |
| GenBank Accession Number | BC002815 |
| Gene Symbol | GAD1 |
| Gene ID (NCBI) | 2571 |
| RRID | AB_2107733 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99259 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GAD1 antibody 10408-1-AP | Download protocol |
| IHC protocol for GAD1 antibody 10408-1-AP | Download protocol |
| IP protocol for GAD1 antibody 10408-1-AP | Download protocol |
| WB protocol for GAD1 antibody 10408-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GAD1 Polyclonal Antibody (Cat# 10408-1-AP) has 46 citations published in journals including Cancer Cell, Cell, Nature Cell Biology, Nature Communications, Theranostics, Science Advances, EMBO Molecular Medicine, and others. Research fields span neuroscience (GABA signaling, Parkinson's disease, depression, pain, autism), oncology (breast, colon, lung, renal, and endometrial cancers), metabolism, toxicology, and cardiology.
| Species | Application | Title |
|---|---|---|
Cancer Cell GABA promotes resistance to immunotherapy in patients with TLS-positive tumors. | ||
Nat Cell Biol Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.
| ||
Nat Commun All-optical reporting of inhibitory receptor driving force in the nervous system | ||
Theranostics The delta subunit of the GABAA receptor is necessary for the GPT2-promoted breast cancer metastasis | ||
Theranostics RPS23RG1 inhibits SORT1-mediated lysosomal degradation of MDGA2 to protect against autism |
Reviews
What customers say
One reviewer (Lana Yablonska) rated this antibody 4/5 stars for Western Blot on human striatal brain tissue at a 1:1000 dilution. She reported detecting the expected ~67 kDa band but noted the presence of non-specific bands. Her protocol used a 4–12% Bis-Tris gradient gel with overnight transfer at 30V, SEA Block for blocking, and an IRDye 800CW goat anti-rabbit secondary antibody at 1:15,000. Overall, the antibody performed adequately but showed some background issues in her application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lana (Verified Customer) (02-06-2020) | Besides 67 kDa non-specific bands are present.SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.
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