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GAD65 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 21760-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

GAD65 Antibody WB mouse brain tissue 21760-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 21760-1-AP( GAD65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

GAD65 Antibody IP rat brain tissue 21760-1-AP

IP Result of anti-GAD65 (IP:21760-1-AP, 3ug; Detection:21760-1-AP 1:300) with rat brain tissue lysate 4000ug.

GAD65 Antibody IHC human colon tissue 21760-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 21760-1-AP( GAD65 antibody at dilution of 1:200 (under 10x lens).

GAD65 Antibody IHC human colon tissue 21760-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 21760-1-AP( GAD65 antibody at dilution of 1:200 (under 40x lens).

GAD65 Antibody IF mouse brain tissue 21760-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 21760-1-AP (GAD65 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue
Positive IP detected in:
rat brain tissue
Positive IHC detected in:
human colon tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:100-1:400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GAD65 fusion protein Ag16251
Full name:
glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Calculated molecular weight:
585aa,65 kDa
Observed molecular weight:
65 kDa
GenBank accession number:
BC126327
Gene ID (NCBI):
2572
Gene symbol
GAD2
Synonyms
GAD 65, GAD2, GAD65, Glutamate decarboxylase 2

Background

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and insulin secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.


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