Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, mouse heart tissue, HEK-293 cells, SKOV-3 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human ovary tumor tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16260-1-AP targets GADD45GIP1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9299 Product name: Recombinant human GADD45GIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC013027 Sequence: AASVRQARSLLGVAATLAPGSRGYRARPPPRRRPGPRWPDPEDLLTPRWQLGPRYAAKQFARYGAASGVVPGSLWPSPEQLRELEAEEREWYPSLATMQESLRVKQLAEEQKRREREQHIAECMAKMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQELLGYQVDPRSARFQELLQDLEKKERKRLKEEKQKRKKEARAAALAAAVAQDPAASGAPSS Predict reactive species |
| Full Name | growth arrest and DNA-damage-inducible, gamma interacting protein 1 |
| Calculated Molecular Weight | 222 aa, 25 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC013027 |
| Gene Symbol | GADD45GIP1 |
| Gene ID (NCBI) | 90480 |
| RRID | AB_2108229 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAE8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GADD45GIP1 antibody 16260-1-AP | Download protocol |
| IP protocol for GADD45GIP1 antibody 16260-1-AP | Download protocol |
| WB protocol for GADD45GIP1 antibody 16260-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GADD45GIP1 Polyclonal antibody (Cat# 16260-1-AP) has 10 citations published in journals including Science, Nature Structural & Molecular Biology, Developmental Cell, Oncotarget, Burns & Trauma, Cancer Cell International, Cell & Molecular Life Sciences, International Journal of Molecular Sciences, Journal of Molecular Medicine, and Journal of Inflammation Research. Research fields span T cell immunometabolism, mitochondrial ribosome assembly, cardiac proteomics, osteosarcoma, lung adenocarcinoma, diabetic nephropathy, hepatocellular carcinoma EMT, cerebral ischemia neuroprotection, and atrial fibrillation.
| Species | Application | Title |
|---|---|---|
Science T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration. | ||
Burns Trauma Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction. | ||
Cancer Cell Int GADD45GIP1 promotes osteosarcoma progression by modulating RPL35 ubiquitination and alleviating endoplasmic reticulum stress via the PERK/eIF2α pathway | ||
Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation |
