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GADL1 Polyclonal antibody

GADL1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 18240-1-AP

Synonyms

GADL1, glutamate decarboxylase like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using GADL1 Polyclonal antibody (18240-1-AP)

    WB analysis of mouse cerebellum using 18240-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18240-1-AP (GADL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GADL1 Polyclonal antibody (18240-1-AP)

    WB analysis of human brain using 18240-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18240-1-AP (GADL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GADL1 Polyclonal antibody (18240-1-AP)

    IP experiment of mouse brain using 18240-1-AP

    IP result of anti-GADL1 (IP:18240-1-AP, 4ug; Detection:18240-1-AP 1:300) with mouse brain tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse cerebellum tissue, human brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18240-1-AP targets GADL1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GADL1 fusion protein Ag13005
Full Name glutamate decarboxylase-like 1
Calculated Molecular Weight 39 kDa, 59 kDa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC093701
Gene Symbol GADL1
Gene ID (NCBI) 339896
RRIDAB_10699874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GADL1 antibody 18240-1-APDownload protocol
IP protocol for GADL1 antibody 18240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols