Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
10072-1-AP targets GAS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0114 Product name: Recombinant human GAS7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 184-382 aa of BC001152 Sequence: EEDYAKNLAKLSQNSLASQEEGSLGEAWAQVKKSLADEAEVHLKFSAKLHSEVEKPLMNFRENFKKDMKKCDHHIADLRKQLASRYASVEKARKALTERQRDLEMKTQQLEIKLSNKTEEDIKKARRKSTQAGDDLMRCVDLYNQAQSKWFEEMVTTTLELERLEVERVEMIRQHLCQYTQLRHETDMFNQSTVEPVDQ Predict reactive species |
| Full Name | growth arrest-specific 7 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC001152 |
| Gene Symbol | GAS7 |
| Gene ID (NCBI) | 8522 |
| RRID | AB_2108090 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60861 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Growth arrest-specific (GAS) genes are expressed preferentially in cells that enter a quiescent state. Gas7, first identified in serum-starved murine fibroblasts, encodes a 48-kDa protein. 3 isoforms of Gas7 were identified in rat brains, with molecular weight of 38, 47 and 55 kDa in SDS Page.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GAS7 antibody 10072-1-AP | Download protocol |
| WB protocol for GAS7 antibody 10072-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomater Sci Dicalcium silicate microparticles modulate the differential expression of circRNAs and mRNAs in BMSCs and promote osteogenesis via circ_1983-miR-6931-Gas7 interaction. | ||
J Proteome Res Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins. | ||
